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This is a short overview of laboratory techniques I have worked with:
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molecular
biology: isolation of DNA and RNA, PCR, southern-blotting, cloning, creation of cRNA probes for in situ hybridization
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biochemistry:
protein extraction,
western-blotting,
immunprecipitation,
preparation of lipid-rafts
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cell
culture: cultivation
of different cell lines,
cryo-conservation, primary
culture of neurons and
lymphocytes, transient
transfections
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in
situ hybridization:
conventional and fluorescent
(FISH)
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analysis
of lymphocytes:
cellular separation with
AutoMACS, FACS-analysis,
Ca-flux-measurements with
LSR-cytometer,
proliferation- and
apoptosis-essays
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immunohistochemistry:
conventional and fluorescent
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applied
behavioral biology:
fear-conditioning, hot-plate
analgesia, open-field,
hole-board, startle-response,
smell-test, taste-novelty
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